Abstract : Indonesian leaffish (Pristolepis grootii) is one of Indonesia's endemic fish species that has the potential to be cultivated. The unavailability of quality seeds is a main obstacle in P. grooti farming. This study aimed to induce gonadal maturation of P. grootii. A total of 95 females (100.81 ± 0.78 g), reared in 15 aquaria (n=9), fed with a commercial pellet, and injected twice on the 7th and 14th day.with PMSG 10 IU.kg-1 of BW (T1), PMSG 10 + LHRHa 50 g.kg-1 of BW (T2), PMSG 20 (T3), PMSG 20 + LHRHa 50 (T4), and control (T5). Blood samples were collected from the caudal vein at 0, 30, and 60 days post-injection. The results showed that P grootii injected with a combination PMSG 20 + LHRHa 50 had the highest estradiol 17β (905.46±83.09 ρg.ml-1), total cholesterol (383.78±40.57 mg.dL-1), GSI (1.68±0.12 %), fecundity (2,946±174.72 egg.fish-1), and oocyte diameter (922.64±11.54 µm). The combination of PMSG 20 IU.kg-1 of BW + LHRHa 50 g. kg-1 of BW was the best treatment for gonadal maturation of P. grootii.