DGAT1 (Diacylglycerol Acyltransferasal1) gene contributes to fatty acid regulation and considered as a potential candidate gene for meat quality. This study aimed to explore the polymorphism of the DGAT1 gene in Indonesian sheep and its association with carcass weight, meat quality, and retail cuts. A total sample of sheep used 169 rams of 10-12 months old from unrelated family and different farms consisted 20 Garut sheep (GS), 35 Compass Agrinak sheep (CAS), 36 Barbados cross sheep (BCS), 20 Javanese fat-tailed (JFT), 17 Javanese thin-tailed (JTT), and 41 Garut composite sheep (GCS). Polymorphism of the DGAT1 gene were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with AluI restriction enzyme. The results of identification found two genotypes namely CC dan CT in GS, JFS, and JTT indicate that polymorphism in the DGAT1 gene. This polymorphism is associated (P<0.05) with characteristics and retail cuts of carcass including the weight of carcass, the weight of breastbone, and weight of subcutaneous breast fat. CC genotype had higher weight in characteristic and retail cuts of carcass than the CT genotype. However, the mutation of DGAT1 was not significantly associated (P>0.05) with meat quality. It could be concluded that polymorphism of DGAT1 contributed to characteristic and retail cuts of the carcass in sheep.