Muna Regency is an area that has been designated as one of the Bali cattle development areas in Southeast Sulawesi. Bali cattle in Muna Regency in recent years have continued to experience an increase in population but this has not been followed by maximum body weight gain. This study aims to analyze soil nutrient content, natural grass nutrition, and its relationship with the body weight gain of Bali cattle in the Muna District. The study used a survey method by taking soil and natural grass samples at each sampling point in 12 study areas. The samples were then analyzed in the laboratory of soil science and feed nutrition at Halu Oleo University, Southeast Sulawesi. The results of the study found that from the 12 study areas, soil nutrients were in the neutral to the slightly alkaline range, which caused natural grasses not to have enough nutrients to grow optimally, while for natural grasses, the highest protein was in the Parigi region, which reached 3.94% and certainly had a relationship with body weight gain because most of the natural grass, which is a source of feed, contains low non-protein nitrogen (NPN). The study concluded that the body weight gain of Bali cattle in Muna Regency did not experience a significant increase due to a lack of non-protein nitrogen (NPN) found in natural grass and possibly due to low soil nutrients.