This research motivated by the need to assemble the resistant to brown plant hoppers black rice varieties whose performance is farmers and the community favor. Many local genetic resources are available for black rice, one of which is Cempo Ireng. Improvements in the characteristics of this rice has been carried out to produce the expected lines of Sebelas Maret University of black rice, but this line is not resistant to brown plant hoppers. Preliminary research has carried out by irradiating the line of black rice using 200 Gy gamma rays. The current research aims to evaluate the early flowering M2 population, observe the performance variability, heritability, lignin content and conduct individual selection for the next generation population. Lignin content was evaluate as an approach to identify resistance to brown plant hoppers. Lignin accumulation is needed for against the brown plant hopper attack on rice because lignin can strengthen the plant tissue and affected to herbivore eat behaviour. The results showed that there were variations in lignin content ranging from 10 to 22%, and high heritability was found in plant height. Wide genetic variability found on plant height and number seed per panicle. This results can be take into consider for individual selection on this generation. We get 20 number of plant accession selected for M3 population.