A series of new pyrazole-containing compounds 3a-c were synthesized from chalcone derivatives 2a-c with an excess of hydrazine hydrate under reflux in acetic acid. The structure of the produced compounds was established by Melting point, TLC, FT-IR spectroscopy, and 1H-NMR. The target compounds (3-acetylcoumarin, 2a, 2b, 2c, 3a, 3b, 3c & Pivaloylampicillin) underwent a docking procedure Ampicillin-CTX-M-15. The orthosteric target was shown to bind to every hit. (Active) location of the enzyme, which might signify a competitive inhibition mechanism. The cytotoxic effect of compound 3b against MCF-7 cells was studied. The antiproliferative activity of compound 3b was tested by studying its ability to inhibit cell proliferation. The results demonstrated the ability of compound 3b to make morphological changes in breast cancer cell lines after being treated with compound 3b.